Primers used for MLST of Mycobacterium abscessus
Genes
The Mycobacterium abscessus MLST scheme uses internal fragments of the following housekeeping genes:
argH (argininosuccinate lyase)
cya (adenylate cyclase)
glpK (glycerol kinase)
gnd (6-phosphogluconate deshydrogenase)
murC (UDP N-acetylmuramate-L-Ala ligase)
pta (phosphate acetyltransferase)
purH (phoshoribosylaminoimiazolcarboxylase ATPase subunit)
Primers for PCR amplification and sequencing
| Gene | Direction | Sequence |
|---|---|---|
| argH | ARGHF: | GACGAGGGCGACAGCTTC |
| ARGHSR1: | GTGCGCGAGCAGATGATG | |
| cya | ACF: | GTGAAGCGGGCCAAGAAG |
| ACSR1: | AACTGGGAGGCCAGGAGC | |
| glpK | GLPKSF1: | AATCTCACCGGCGGTGTC |
| GLPKSR2: | GGACAGACCCACGATGGC | |
| gnd | GNDF: | GTGACGTCGGAGTGGTTGG |
| GNDSR1: | CTTCGCCTCAGGTCAGCTC | |
| murC | MURCSF1: | CGGACGAAAGCGACGGCT |
| MURCSR2: | CCAAAACCCTGCTGAGCC | |
| pta | PTASF1: | GATCGGGCGTCATGCCCT |
| PTASR2: | ACGAGGCACTGCTCTCCC | |
| purH | PURHSF1: | CGGAGGCTTCACCCTGGA |
| PURHSR2: | CAGGCCACCGCTGATCTG |
PCR conditions
Conditions for PCR amplification are 5 min at 94°C followed by 30 cycles of 94°C for 1 min, 58°C for 1 min and 72°C for 1 min with a final extension step at 72°C for 10 min.
Allele templates
Please search allele 1 from the sequence download page