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The BIGSdb website is implementing new settings regarding data access. Data curated in 2025 (and later) will not be accessible for anonymous (non-logged-in users). If you wish to have access to data curated in 2025 and beyond, please register on the platform and login when using it. Note that access via the application programming interface (API) will also require authentication to access recent data (API authentication help link).

To ensure a smooth transition, anonymous data access will be periodically closed and opened from 1st October 2024 until the end of the year. For detailed planning, please follow this link.

On 1st January 2025, registration will be permanently mandatory to access data curated after the 31st of December 2024.

Please contact us if you have any questions.

Primers used for MLST of Mycobacterium abscessus

Genes

The Mycobacterium abscessus MLST scheme uses internal fragments of the following housekeeping genes:

argH (argininosuccinate lyase)
cya (adenylate cyclase)
glpK (glycerol kinase)
gnd (6-phosphogluconate deshydrogenase)
murC (UDP N-acetylmuramate-L-Ala ligase)
pta (phosphate acetyltransferase)
purH (phoshoribosylaminoimiazolcarboxylase ATPase subunit)

Primers for PCR amplification and sequencing

GeneDirectionSequence
argHARGHF:GACGAGGGCGACAGCTTC
ARGHSR1:GTGCGCGAGCAGATGATG
cyaACF:GTGAAGCGGGCCAAGAAG
ACSR1:AACTGGGAGGCCAGGAGC
glpKGLPKSF1:AATCTCACCGGCGGTGTC
GLPKSR2:GGACAGACCCACGATGGC
gndGNDF:GTGACGTCGGAGTGGTTGG
GNDSR1:CTTCGCCTCAGGTCAGCTC
murCMURCSF1:CGGACGAAAGCGACGGCT
MURCSR2:CCAAAACCCTGCTGAGCC
ptaPTASF1:GATCGGGCGTCATGCCCT
PTASR2:ACGAGGCACTGCTCTCCC
purHPURHSF1:CGGAGGCTTCACCCTGGA
PURHSR2:CAGGCCACCGCTGATCTG

PCR conditions

Conditions for PCR amplification are 5 min at 94°C followed by 30 cycles of 94°C for 1 min, 58°C for 1 min and 72°C for 1 min with a final extension step at 72°C for 10 min.

Allele templates

Please search allele 1 from the sequence download page

Edit on GitLab