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Primers used for MLST ofย Mycobacteriumย abscessus
Genes
The Mycobacterium abscessus MLST scheme uses internal fragments of the following housekeeping genes:
argH (argininosuccinate lyase)
cya (adenylate cyclase)
glpK (glycerol kinase)
gnd (6-phosphogluconate deshydrogenase)
murC (UDP N-acetylmuramate-L-Ala ligase)
pta (phosphate acetyltransferase)
purH (phoshoribosylaminoimiazolcarboxylase ATPase subunit)
Primers for PCR amplification and sequencing
Gene | Direction | Sequence |
---|---|---|
argH | ARGHF: | GACGAGGGCGACAGCTTC |
ARGHSR1: | GTGCGCGAGCAGATGATG | |
cya | ACF: | GTGAAGCGGGCCAAGAAG |
ACSR1: | AACTGGGAGGCCAGGAGC | |
glpK | GLPKSF1: | AATCTCACCGGCGGTGTC |
GLPKSR2: | GGACAGACCCACGATGGC | |
gnd | GNDF: | GTGACGTCGGAGTGGTTGG |
GNDSR1: | CTTCGCCTCAGGTCAGCTC | |
murC | MURCSF1: | CGGACGAAAGCGACGGCT |
MURCSR2: | CCAAAACCCTGCTGAGCC | |
pta | PTASF1: | GATCGGGCGTCATGCCCT |
PTASR2: | ACGAGGCACTGCTCTCCC | |
purH | PURHSF1: | CGGAGGCTTCACCCTGGA |
PURHSR2: | CAGGCCACCGCTGATCTG |
PCR conditions
Conditions for PCR amplification are 5 min at 94ยฐC followed by 30 cycles of 94ยฐC for 1 min, 58ยฐC for 1 min and 72ยฐC for 1 min with a final extension step at 72ยฐC for 10 min.
Allele templates
Please search allele 1 from the sequence download page