Whole genome sequencing data requirements

Users are requested to submit only high-quality assemblies, generated from pure cultures sequenced at a minimum coverage of 40X. Assembly files consisting of high numbers of contigs (> 600), presenting a cumulative contigs length outside the typical range of Leptospira (~ 3.5 - 6 Mbp) will not be accepted. Submissions containing low quality assemblies may be entirely rejected.

NB. Please note that new alleles and profiles will not be defined based on long-read sequence technology alone nor based on Ion Torrent/Roche/454, to avoid introducing artifact sequences in the database due to low accuracy sequencing. For typing purposes, we recommend using only assemblies either generated from high quality short-reads or combining both short and long reads (hybrid assemblies).

Please refer to the assembly metrics below: